Recombinant expression, intrabody, immunomodulation

Express and purify recombinant nanobodies in large amounts (10-100 mg, ormore) at relatively low cost in bacteria or yeast. You routinely obtain milligram quantities per liter bacterial culture. Now you have your pet antibodies for the next decade, and longer. Add any tag you like. Remember, you have the nanobody cDNA! Depending on the epitope they recognize, they can be useful for Western blotting, pull-down/immunoprecipitation experiments. No need to repeatedly purchase antibodies. Let bacteria do the work for you. Use nanobodies like any other cDNA: clone it into an expression vector and transform, transfect, transduce, nucleofect, electroporate… your nanobody into a prokaryotic host or into eukaryotic cells and modulate properties of the antigen. Alternatively, you can use protein transduction to introduce a recombinant nanobody directly into cells. Learn the affinity of the nanobody-antigen interaction via SPR, isothermal titration calorimettry, Octet platform…. Epitope mapping becomes easy: using antigen deletion fragments, NMR, X-ray crystallography,..